Interaction of Vibrio cholerae toxin with sarcoma 180 cell membranes.Wheeler MA,Solomon RA,Cooper C,Hertzberg L,Mehta H,Miki N,Bitensky MW.Three discrete phases are discernible in the activation,by Vibrio cholerae toxin,of adenylate cyclase in fragments of sarcoma 180 cell membranes. results for this questionHow are biofilms used to study Vibrio cholerae?How are biofilms used to study Vibrio cholerae?Here,we use an optical imaging technique to visualize every cell inside thousands of Vibrio cholerae biofilms to discover architectural transitions and the major phases of V.cholerae biofilm growth.Architectural transitions in Vibrio cholerae biofilms at results for this questionWhat is accessory cholera enterotoxin?What is accessory cholera enterotoxin?Additionally,accessory cholera enterotoxin (Ace) causes fluid secretion in ligated rabbit ileal loops ( Trucksis et al.,1993) while cholix toxin (ChxA) is an exotoxin which has been characterized as a member of the eukaryotic elongation factor 2-specific ADP-ribosyltransferase toxins ( König et al.,2016 ).V.Frontiers Accessory Toxins of Vibrio Pathogens and Their
results for this questionWhat is the mechanism of action of cholera?What is the mechanism of action of cholera?DETECTION OF CHOLERA TOXIN A.MODE OF ACTION OF CHOLERA TOXIN The production of cholera toxin (CT) is an essential virulence property of epidemic strains of Vibrio choleraeO1.Each CT molecule is composed of five B (binding) subunits and one A (active) subunit.The B subunits bind to G M1Chapter 7 Detection of Cholera ToxinAntibodies Enhance Interaction of Vibrio cholerae with
Dec 01,2006·Bacteria,cell line,culture conditions,and reagents.The bacterial strains used in this work were Vibrio cholerae LPB1,a transcytosis-proficient laboratory collection strain derived from O395,and the cholera toxin (CT) mutant V.cholerae O395NT with a deletion/insertion of a kanamycin cassette at the ctxAB locus,which was kindly provided by J.J.Mekalanos ().Antibodies enhance interaction of Vibrio cholerae with Sep 25,2006·Our present result suggests that the interaction of sIgA-opsonized V.cholerae with membrane-associated GM 1 is more sensitive to exogenous CTB than is the interaction of nonopsonized V.cholerae with M-like cells and GM 1.One hypothesis that explains our findings is that GM 1 may be working as coreceptor for antibodies.
Apr 05,2016·Fig.1.V.cholerae wild-type biofilm at single-cell resolution.(A) Planar cross-sections through the biofilm at heights z = 0.6 µm,12.6 µm,and 24.6 µm.(B) All individual cells from A were automatically segmented into separate 3D objects,which are color-coded in panel B according to the heights of their centers of mass using the color scale indicated at the top right.Cell Vacuolation,a Manifestation of the El Tor Hemolysin Culture supernatants of nontoxigenic nonepidemic clinical strains of Vibrio cholerae belonging to diverse serogroups were found to induce vacuolation of nonconfluent HeLa cells.The vacuoles became prominent 18 h after introduction of culture supernatant,and vacuolated cells survived for 48 h and then died.Only a fraction of the vacuolated cells took up neutral red dye,implying that there Cited by 11Publish Year 1975Author D A HartInteractions of choleragenoid and GM1 ganglioside with Apr 01,1976·The heat-labile enterotoxins of Vibrio cholerae and Escherichia coli induce morphologic changes and steroidogenesis in clonal lines of adrenal tumor cells in tissue culture; these effects are preventable by prior incubation of either toxin with GM1 ganglioside (galactosyl-N-acetylgalactosaminyl [sialosyl] lactosyl ceramide; GGnSLC) but are not preventable by prior incubation of adrenal cells
Cited by 13Publish Year 1976Author M.A.Wheeler,R.A.Solomon,C.Cooper,L.Hertzberg,H.Mehta,N.Mild,M.W.BitenskyAspects of the interaction of Vibrio cholerae toxin with
Aspects of the interaction of Vibrio cholerae toxin with the pigeon red cell membrane.King CA,van Heyningen WE,Gascoyne N.The ganglioside galactosy-N-acetylgalactosaminyl [sialosyl] lactosyl ceramide (GM1) is readily accumulated in pigeon red cell membranes soaked with [3H]GM1 (1-100 mug/ml) at 37 C for 30 min.Cited by 14Publish Year 2018Author Diliana Pérez-Reytor,Victor Jaña,Leonardo Pavez,Paola Navarrete,Katherine GarcíaMechanism of activation of adenylate cyclase by Vibrio Mechanism of activation of adenylate cyclase by Vibrio cholerae enterotoxin.Published. but also in avian erythrocytes,rat fat cells and cultured melanoma cells.When cholera toxin is incubated with intact cells it stimulates adenylate cyclase activity,as measured in the subsequently isolated plasma membranes,according to a triphasic Cited by 157Publish Year 1973Author Nathaniel F.PierceCytotoxic Cell Vacuolating Activity from Vibrio cholerae ·Gastrointestinal episodes associated with Vibrio species have been rising worldwide in the last few years.Consequently,it is important to comprehend how occurs the production of diarrhea,to establish new preventive and therapeutic measures.Besides the classical CT and TCP toxins,Zot,RTX,and Ace among others have been deeply studied in V.cholerae.
Cited by 157Publish Year 1973Author Nathaniel F.PierceVibrio cholerae-induced cellular responses of polarized
To study the utility of in vitro-polarized intestinal cell monolayers for modeling Vibrio cholerae-host cell interactions,we added live V.cholerae bacteria to the apical surfaces of polarized T84 cell monolayers and monitored changes in electrical properties.We found that both classical and El ToCited by 16Publish Year 2006Author Luz P.Blanco,Victor J.DiRitaEvidence Non-Protein Nature of Receptor for Enterotoxin The enterotoxin of Vibrio cholerae has been shownto affect themetabolic activity ofseveral cell types (reviewed in 6) including lymphoid cells (2,8,17; D.A.HartandR.A.Finkelstein,J.Immunol.,in press).The mechanism of action of the enterotoxin (choleragen) is be-lieved to be the activation ofmembrane-bound adenylate cyclase after Cited by 16Publish Year 2006Author Luz P.Blanco,Victor J.DiRitaPathophysiological effects of Vibrio cholerae and Sep 01,1978·Cuatrecasas P.Interaction of Vibrio cholerae enterotoxin with cell membranes.Biochemistry.1973 Aug 28; 12 (18):35473558.Cuatrecasas P.Vibrio cholerae choleragenoid.Mechanism of inhibition of cholera toxin action.Biochemistry.1973 Aug 28; 12 (18):35773581.Cuatrecasas P,Parikh I,Hollenberg MD.
Cited by 16Publish Year 2013Author Srikar Reddy,Michael Taylor,Mojun Zhao,Patrick Cherubin,Sandra Geden,Supriyo Ray,David FrancisEnterotoxin and Exotoxin Encyclopedia
The channel allows the B subunit to get into the cell.An example of an A-B toxin is the enterotoxin that is produced by Vibrio cholerae.The cholera toxin disrupts the ionic balance of the host's intestinal cell membranes.As a result,the cells of the small intestine exude a large amount of water into the intestine.Cited by 19Publish Year 2011Author Tanaya Chatterjee,Debadrita Mukherjee,Sucharita Dey,Aritrika Pal,Kazi Mirajul Hoque,Pinak ChakrGrape extracts inhibit multiple events in the cell biology Abstract.Vibrio cholerae produces cholera toxin (CT),an AB5 protein toxin that is primarily responsible for the profuse watery diarrhea of cholera.CT is secreted into the extracellular milieu,but the toxin attacks its Gs target within the cytosol of a host cell.Thus,CT must cross a cellular membrane barrier in order to function.Cited by 20Publish Year 1976Author Carolyn A.King,William E.van Heyningen,Norman GascoyneInteractions of choleragenoid and GM1 ganglioside with The heat-labile enterotoxins of Vibrio cholerae and Escherichia coli induce morphologic changes and steroidogenesis in clonal lines of adrenal tumor cells in tissue culture; these effects are preventable by prior incubation of either toxin with GM1 ganglioside (galactosyl-N-acetylgalactosaminyl [sialosyl] lactosyl ceramide; GGnSLC) but are not preventable by prior incubation of adrenal cells with choleragenoid.
V.cholerae O1 should be confirmed.NonO1 - V.cholerae strains may produce CT or other toxins such as heat-stable enterotoxin or Shiga-like toxin,but these strains are very rare and have not been associated with epidemic disease.Therefore,there seems to be little public health benefit in testing sporadic isolates of nonO1 V.- choleraeCited by 23Publish Year 1976Author Sam T.DontaVibrio cholerae [email protected]Vibrio cholerae Subject Areas on Research Cited by 634Publish Year 1973Author Pedro CuatrecasasInteraction of Vibrio cholerae enterotoxin with cell Aug 28,1973·1.Biochemistry.1973 Aug 28;12(18):3547-58.Interaction of Vibrio cholerae enterotoxin with cell membranes.Cuatrecasas P.PMID 4731191 [PubMed - indexed for MEDLINE]
Cited by 634Publish Year 1973Author Pedro CuatrecasasPeople also askWhat is the diagnosis of Vibrio cholerae?What is the diagnosis of Vibrio cholerae?Laboratory Methods for the Diagnosis of Vibrio cholerae Centers for Disease Control and Prevention VII.DETECTION OF CHOLERA TOXIN A.MODE OF ACTION OF CHOLERA TOXIN The production of cholera toxin (CT) is an essential virulence property of epidemic strains of Vibrio choleraeO1.Chapter 7 Detection of Cholera ToxinCited by 7Publish Year 1975Author R.M.Wishnow,E.Lifrak,C.C.ChenAccessory Cholera Enterotoxin,Ace,from Vibrio cholerae
Vibrio cholerae accessory cholera enterotoxin (Ace) is the third toxin,along with cholera toxin (CT) and zonula occludens toxin (Zot),that causes the endemic disease cholera.Structural characterization of Ace has been restricted because of the limited production of this toxic protein by V.cholerae.We have cloned,overexpressed,and purified Ace from V.cholerae strain O395 in Escherichia coli toCited by 93Publish Year 2001Author Karla Jean Fullner,Wayne I.Lencer,Wayne I.Lencer,John J.MekalanosEffect of cholera enterotoxin on ion transport across In vitro effects of cholera toxin correlated closely with in vivo effects heating toxin destroyed both; two V.cholerae filtrates which were inactive in vivo proved also to be inactive in vitro; PD and volume flow measurements in isolated,in vivo ileal loops of rabbit revealed that the PD pattern after addition of toxin is similar to that seen in vitro and also correlates closely with changes in fluid movement.
Natural cholera toxoid appears to act as a competitive inhibitor of cholera enterotoxin and is thus a useful tool for studying the interaction of cholera enterotoxin with cell membranes.Cholera enterotoxin binds to gut mucosa more rapidly than does its natural toxoid.Once binding occurs,however,it appears to be prolonged for both materials.Formalinized cholera toxoid has no inhibitory effect upon choleraDifferential inhibitory effects of cholera toxoids and Apr 01,1973·Natural cholera toxoid appears to act as a competitive inhibitor of cholera enterotoxin and is thus a useful tool for studying the interaction of cholera enterotoxin with cell membranes.Cholera enterotoxin binds to gut mucosa more rapidly than does its natural toxoid.Once binding occurs,however,it appears to be prolonged for both materials.File Size 740KBPage Count 27Interaction of cholera enterotoxin with cultured adrenal Abstract.In the adrenal tumor cell system ganglioside Gm1 inhibited cholera enterotoxin (CT)-induced steroidogenesis if it was preincubated with the toxin or added to adrenal cells 10 min before CT.In the preincubation studies a molar ratio of Gm1 to toxin of 3:1 was necessary for half-maximal inhibition of steroidogenesis.
Vibrio cholerae,a noninvasive enteropathogen and the etiological agentofcholera,adheresto epithelial cells ofthe proximal small intestine and excretes a potent enterotoxin whichcausesseverediarrhea.Severalmodelssimulatingthe important features ofthe interaction betweenthe bacterium andtheepithelial surfacehavebeenproposed(8,11,15,26).Interaction of Vibrio cholerae enterotoxin with cell Affinity chromatography and structural analysis of Vibrio cholerae [Vibrio Comma] enterotoxin-ganglioside agarose and the biological effects of ganglioside-containing soluble polymers.Biochemistry 1973,12 Cholera toxin-fat cell interaction and the mechanism of activation of the lipolytic response.Biochemistry 1973,12 (18) ,Some results are removed in response to a notice of local law requirement.For more information,please see here.12345Next
Vibrio cholerae is the causative agent of the diarrheal disease cholera.Although many V.cholerae virulence factors have been studied,the role of interbacterial interactions within the host gut and their influence on colonization are poorly understood.Here,we utilized the conjugative properties